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Laboratory standard operating procedure for MLVA of Salmonella enterica serotype Typhimurium

23 Sep 2011
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Abstract

This report describes the standardised laboratory protocol for molecular subtyping of Salmonella enterica serotype Typhimurium. It is based on the 5-locus multiple-locus variable-number tandem repeat analysis (MLVA) method.

This technical document provides a single protocol for performing MLVA of S. Typhimurium and thereby ensure inter-laboratory comparability of the generated results using capillary electrophoresis.

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