Laboratory standard operating procedure for MLVA of Salmonella enterica serotype Typhimurium

protocol technical guidance

This report describes the standardised laboratory protocol for molecular subtyping of Salmonella enterica serotype Typhimurium. It is based on the 5-locus multiple-locus variable-number tandem repeat analysis (MLVA) method.
This technical document provides a single protocol for performing MLVA of S. Typhimurium and thereby ensure inter-laboratory comparability of the generated results using capillary electrophoresis.